Mycobacterial species as case-study of comparative genome analysis

Recently, the availability of bioinformatics tools for genomic comparison facilitated handling, visualizing and analyzing of enormous amount of sequence information of multispecies bacterial genomes [ 2930 ]. It is noteworthy that the human TB could be also induced by M.

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As the degree distribution of the resulting network has also been shown in Figure 3it exhibits scale-free property like many biological networks in which the degree distribution of proteins approximates a power lawwith the degree exponent. Comparing the ESX loci of M.

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Indeed, the efforts worldwide focus on the combat against TB, leprosy, Buruli ulcer and other mycobacterial diseases and the medical care providers face a great challenge toward the achievement of this goal. Retrieval of other sequences. Carbonic anhydrase genes have been numbered according to genomic position.

Mycobacterium fortuitum and to a lesser extent M. Materials and Methods Bacterial Cultures M. Since the characteristic path length of the generated network is 3. As a result of multiple genome analysis, the pan and core genome will be defined for some of Mycobacterial species, which is extremely useful to investigate the true diversity within and between bacterial species 15, Interestingly, the rpoBsequence of one strain Kan among the 32 clinical isolates ofM.

Nucleotide sequence variations of rpoB among the clinical isolates were observed. More recently, Fillol et al. Further, the study of Mycobacterium tuberculosis virulence is another path which has got much attention in the design of drugs with a new mechanism of action, the production of modern concepts, and tuberculosis treatment schemes [ 27 ].

The betweenness centrality measure is a means to quantify the influence of a node in the interaction network. In this study we analyzed the sequences of 20 genes and pseudogenes 22, nucleotides.

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A total of genes were identified as essential genes from the analysis. These two species were previously identified and characterized as novel NTM Gcebe et al. The candidate lists have been classified into their high level functional class and the distribution has been indicated in the diagram.

Slowly growing, pathogenic M. Since it was previously shown by hybridization experiments that ESX-5 might be absent in Mycobacterium nonchromogenicum we verified the presence of esxN ortholog situated in ESX-5 locus in M.

Importantly, most of the pathogenic or slow growing mycobacteria are sharing a high similarity and a strong phylogeny relationship [ 1213 ] and interestingly, several studies confirmed that the pathogenic mycobacteria were originated from a free living progeny [ 9 ] and due to the genome reduction and the acquisition of new genes by horizontal gene transfer HGT [ 14 - 17 ] and gene rearrangement [ 17 ], their capacity of parasitism and infectiousness was developed for enabling them to cause severe and dangerous illnesses.

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LXand V, respectively, were used for comparisons. The comparative genome analysis has helped in identifying those lists of proteins which were stated as essential for the survival and growth of the pathogen to increase success rate of drugs to be designed.

Most of these genes either encode enzymes of several lipid pathways, cell surface proteins, regulators, and proteins of signal transduction systems or are involved in mycobacterial survival inside the aggressive microenvironment of the host macrophages. Without knowledge of the specific sequences of this organism, the primers were designed using the conserved sequences of the corresponding genes of M.

Also, the genomes of two M. It shows that an important node lies on a high proportion of paths between other nodes in the interaction network. Peptide identifications were accepted if they could be established at greater than 9. Summary of the de novo assembly results.

Taken together, the results described above indicate that M.

And for examining the phylogenetic relationships among these bacteria, a phylogentic tree has been constructed from 16S rRNA gene, for tuberculosis and non tuberculosis Mycobacteria to understand the evolutionary events of these species, as stated by other authors 4, Recently, by comparing the genomes of M.

As a result of those studies, M.Comparative sequence analysis of rpoB DNAs provided the basis for species differentiation within the genusMycobacterium. Slowly and rapidly growing groups of mycobacteria were clearly separated, and each mycobacterial species was differentiated as a distinct entity in the phylogenetic tree.

Further, with more than Mycobacterial genome sequences, comparative genome analysis provides exceptional new insights into the biology of these worldwide important pathogens.

In your seventeen years mycobacterial species as case study of comparative genome analysis this earth, the program you are applying to may require a transcript regardless of the number of mycobacterial species as case study of comparative genome analysis credits youve earned.

Identification and comparative analysis of a genomic island in Mycobacterium avium subsp. hominissuis. is lacking this GI and must have virulence-associated genes at other genome sites. Among the species showing the highest homologies to parts of the GIs were M. xenopi. Comparative Sequence Analysis of Mycobacterium leprae and the New Leprosy-Causing This study revealed significant sequence differences between M.

lepromatosis and all known Mycobacterium species and placed M. lepromatosis The extraction was performed using the Microbial Genome Database for Comparative Analysis.

The genomes used. In this present study, we aim to highlight a comparative genome, proteome and phylogeny analysis between twenty-one mycobacterial (Tuberculosis and non tuberculosis) strains using a set of computational and bioinformatics tools (Pan and Core genome plotting, BLAST matrix and.

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Mycobacterial species as case-study of comparative genome analysis
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